Laboratory Techniques

In Laboratory Techniques we gather a wide expertise in a high variety of techniques:

  • Cellular and immunological techniques:
    • Purification and cryopreservation of mononuclear cells from peripheral blood (PBMC).
    • Obtaining cellular subpopulations (lymphocytes TCD4+, lymphocytes TCD8+, monocytes, dendritic cells, Treg…).
    • Immortalization of B-lymphocytes.
    • Flow cytometry.
    • ELISPOT

     

  • Molecular techniques:
    • DNA extraction from peripheral blood mononuclear cells (PBMC) and other cellular subpopulations and cell lines.
    • DNA/RNA/protein extraction from tissue (frozen and paraffin-embedded).
    • Amplification of genomic DNA.

     

  • Techniques related with antiretroviral drugs:
    • Screening and characterization of the anti-HIV activity of compound collections.
    • Phenotypic resistance of antiretroviral drugs.
    • Phenotypic characterization of viral tropism in clinical samples before starting treatment with CCR5 inhibitors.
    • Microbicides evaluation in in vitro models or in small animal models.

     

  • Specific techniques in vaccine research:
    • Titration of neutralizing antibodies against HIV for the assessment of efficacy of new vaccines.
    • Assessment of immunogen prototypes in in vitro models or in small animal models.

     

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